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You need to identify which part of your system that is slow and has the biggest impact, which is only visible when you put your site under actual load. What I usually suggest is to have load test against a site in an actual configuration, and use tools like Azure Application Insights or New Relic to see how the site performs. After you know which part is slow, tools like dotTrace can give more detailed information on that part, but not before
We used dottrace, prefix and ANTS profiler but its not giving any information in the controller, model(page type or block type) and view area that needs further investigation and not even in business layer.
Is there any profiling tool that will give these information?